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GLYCOPRO
Sigma
Glycoprotein Detection Kit
Description
| Application | The Glycoprotein Detection Kit is designed for the selective staining of glycoproteins on polyacrylamide gels and membranes using a modification of the Periodic Acid-Schiff (PAS) method.1 Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The periodic acid/Schiff reagent stains vicinal diol groups found mainly on peripheral sugars and sialic acids and is used as a general glycoprotein stain.2 |
Properties
| usage | kit sufficient for 10 mini-gels (10 × 10 cm) |
| kit sufficient for 5 gels or membranes (16.5 × 19 cm) | |
| measuring range | 25-100 ng/band carbohydrate detection limit |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | O,C |
| Risk Statements | 8-22-31-34-41 |
| Safety Statements | 17-26-36/37/39-45-46 |
| RIDADR | UN 3316 9/PG 2 |
Components
| Standard component | Horseradish Peroxidase (Positive control) |
| Oxidation component (Periodic Acid) | |
| Reduction component (Sodium Metabisulfite) | |
| Schiff’s reagent, Fuchsin-sulfite reagent |
References
| Cited Reference | 1. Zacharius, R.M., et al., Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem. 31, 148–152, (1969) |
| 2. Thornton, D.J, et al., Walker, J.M., ed., Identification of glycoproteins on nitrocellulose membranes and gels. Methods Mol. Biol. Totowa, NJ , (1994) 32, 119-128 | |
| reference | , Andrews, A.T., ed., Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications. Biochemical and Clinical Applications, 2nd ed, (1988), 38-42 |
Jay, G.D., et al., Silver staining of extensively glycosylated proteins on sodium dodecyl sulfate-polyacrylamide gels: enhancement by carbohydrate-binding dyes. Anal. Biochem. 185, 324-330, (1990) Abstract | |
| Devine, P.L. and Warren, J.A., Glycoprotein detection on Immobilon PVDF transfer membrane using the Periodic Acid/Schiff Reagent. Biotechniques 8, 492, (1990) Abstract |
